Functional characterization of a vascularized 3D bioartificial liver

Functional characterization of a vascularized 3D bioartificial liver with perfused human precision-cut liver slices in a microfluidic culture system as standard
By PhD Student Viktoriia Starokozhko , RUG

The objective of this PhD project is to optimize the viability of human precision-cut liver slices (PCLS) cultured in a perfusion-based microfluidic microchamber, to be used as benchmark for testing of the functionality of the newly engineered BAL. In brief, our aims are:

  • to optimize the viability and metabolic activity of human PCLS incubated in a biochip beyond 48h  
  • to assess viability, oxidation status and function of PCLS by ATP content, enzyme leakage, hypoxy staining, glycogen staining, measurement of drug metabolism, ammonia elimination, bilirubin and bile salt production
  • to perform functional characterization of BAL and compare it with organ-derived liver slices
  • to assess overall functionality and viability of newly developed BAL

Partners involved in this project is:

https://www.nanobio4trans.eu/technology/projects/functional_characterization_of_a_vascularized_3d_bioartificial_liver
5 JUNE 2020