Functional characterization of a vascularized 3D bioartificial liver with perfused human precision-cut liver slices in a microfluidic culture system as standard
By PhD Student
Viktoriia Starokozhko
, RUG
The objective of this PhD project is to optimize the viability of human precision-cut liver slices (PCLS) cultured in a perfusion-based microfluidic microchamber, to be used as benchmark for testing of the functionality of the newly engineered BAL. In brief, our aims are:
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to optimize the viability and metabolic activity of human PCLS incubated in a biochip beyond 48h
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to assess viability, oxidation status and function of PCLS by ATP content, enzyme leakage, hypoxy staining, glycogen staining, measurement of drug metabolism, ammonia elimination, bilirubin and bile salt production
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to perform functional characterization of BAL and compare it with organ-derived liver slices
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to assess overall functionality and viability of newly developed BAL
Partners involved in this project is: